Download Antiviral Methods and Protocols by Derek Kinchington, Raymond F. Schinazi PDF

By Derek Kinchington, Raymond F. Schinazi

ISBN-10: 0896035611

ISBN-13: 9780896035614

Royal London tuition of medication, united kingdom. moment writer, Raymond F. Schinazi, is at Emory Univ., Decatur, GA. bargains easy and medical researchers versions to judge compounds potent opposed to acute and protracted infections. comprises finished assays, caliber checking out, and unpublished tools. DNLM: Hepatitis, Viral, Human - drug treatment.

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Greater sensitivity can be obtained with the culture method, which has a theoretical detection threshold of 1 CFU/mL of sample for cultivable species. However, in order to achieve this level of sensitivity, a culture period of 4 wk is necessary; this should be combined with the use of a second, more sensitive test, such as PCR or culture. In addition, cultures should be tested every time they are recovered from nitrogen storage since mycoplasma may proliferate more quickly than animal cells. Economic factors are clearly important when selecting a test method.

2% hybridization. 4% hybridization. 2% hybridization the results are still valid. 7. 1. Introduction The kit is based on the ELISA technique and contains polyclonal antibodies for the detection of four species of mycoplasma found in contaminated cell cultures (M. orale, M. hyorhinis, M. arginini, A. laidlawii). 36 Stacey and Stacey All reagents are supplied with the kit and are stored at 4°C; however, all reagents should have reached room temperature (18–25°C) before use. 1. 25 mL of each capture antibody (one for each mycoplasma species) into two wells for each sample and into one well for each control, according to the information in Table 1.

Wise, K. , Cassell, G. , and Action, R. T. (1978). Selective association of murine T lymphoblastoid cell surface alloantigens with M. hyorhinis. Proc. Natl. Acad. Sci. USA 75, 4479–4483. 8. O’Brien, S. , and Shannon, J. E. (1977) Enzyme polymorphisms as genetic signatures in human cell cultures. Science 195, 1345–1348. 9. Jeffreys, A. , and Thein, S. L. (1985) Individual-specific ‘fingerprints’ of human DNA. Nature 316, 76–79. 10. Stacey, G. , Bolton, B. , and Doyle A. (1992) DNA fingerprinting transforms the art of cell authentication.

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