By Christine Kurstak, A. Hossain, Edouard Kurstak (auth.), Edouard Kurstak, R. G. Marusyk, F. A. Murphy, M. H. V. Van Regenmortel (eds.)
Volume three is dedicated to the newest diagnostic expertise for virus illnesses, fairly molecular methodologies.
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Extra resources for Applied Virology Research: New Diagnostic Procedures
However, the latter approach can be compromised by the presence of pseudogenes or incompletely processed mRNAs. The major product of the 3SR reaction is a single-stranded RNA. While the electrophoretic assays for the 3SR RNA product require denaturing conditions for accurate size measurements and thus are more cumbersome than for the DNA product of PCR amplifications, the single-stranded RNA of the 3SR reaction has two advantageous features. , 1991). , 1990). Second, the single-stranded 3SR TRANSCRIPTION-BASED AMPLIFICATION SYSTEMS 47 product is amenable to rapid detection, genotypic characterization and quantitation using bead-based sandwich hybridization (BBSH) reactions.
This process continues in a selfsustained cyclic fashion under isothermal conditions (42°C) until components in the reaction become limiting or inactivated (enzymes). , 1991); circles, reverse transcriptase; diamonds, T7 RNA polymerase; TCS, target complementary sequence. 40 D. Y. R. GINGERAS C. , 1990a; Kwoh and Kwoh, 1990; Gingeras and Kwoh, 1991; Persing, 1991). Interestingly, the 3SR amplification reaction has several features which distinguish it from other methods for nucleic acid amplification.
Sera from 99 volunteer blood donors, from a HN-1low prevalence area of the United States, with the above serologic outcome were coded and tested for the presence of HN by culture and PCR. Ninety-eight of the 99 blood donors had no identified risk factors for HN-1 infection whereas one donor had used intravenous drugs. After a median of 14 months from the time of the initial serologic testing, 65 donors (66%) were still repeatedly reactive for HN-1 on at least one immunoassay. In 91 donors (92%), the Western blot results remained indeterminate.